Analysis of Heme Structural Heterogeneity in Mycobacterium tuberculosis Catalase-Peroxidase (KatG)
نویسندگان
چکیده
منابع مشابه
Structural characterization of recombinant catalase-peroxidase from Mycobacterium tuberculosis.
It has long been observed that resistance to isoniazid (INH), the core compound used to treat tuberculosis, is often correlated with reduced levels of catalase activity [1,2]. It has also been confirmed that the presence of active catalaseperoxidase (CP), encoded by the katG gene, is necessary for INH sensitivity in M. tuberculosis [3]. Point mutations or deletions in katG can give rise to clin...
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Mycobacterium tuberculosis KatG catalyzes the activation of the antitubercular agent isoniazid to yield an inhibitor targeting enoyl reductase (InhA). However, no firm biochemical link between many KatG variants and isoniazid resistance has been established. In the present study, six distinct KatG variants identified in clinical Mycobacterium tuberculosis isolates resistant to isoniazid were ge...
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Background The mycobacterial catalase-peroxidases protect it from the reactive oxidative metabolites and allow its survival within the host phagocytes. We compared the virulence of Mycobacterium avium complex (MAC) clinical isolates with varying catalase activity recovered from the blood of HIV patients in terms of invasiveness and intracellular multiplicity in host cells. The catalase activity...
متن کاملAccess channel residues Ser315 and Asp137 in Mycobacterium tuberculosis catalase-peroxidase (KatG) control peroxidatic activation of the pro-drug isoniazid.
Peroxidatic activation of the anti-tuberculosis pro-drug isoniazid by Mycobacterium tuberculosis catalase-peroxidase (KatG) is regulated by gating residues of a heme access channel. The steric restriction at the bottleneck of this channel is alleviated by replacement of residue Asp137 with Ser, according to crystallographic and kinetic studies.
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†Departamento de Quı́mica, Universidade Federal de Lavras—UFLA, Campus Universitário, Lavras, Caixa Postal 3037, Lavras, MG CEP: 37200-000, Brazil ‡Centro Brasileiro de Pesquisas Fı́sicas (CBPF), Rua Dr. Xavier Sigaud, 150, Urca, Rio de janeiro, RJ 22290-180, Brazil {Departamento de Quı́mica Orgânica, Instituto de Quı́mica, Universidade Federal do Rio de Janeiro, Ilha do Fundão, CT, Bl. A, Lab. 609...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2003
ISSN: 0021-9258
DOI: 10.1074/jbc.m208256200